The Regenerative Potential of Notochordal Cells in a Nucleus Pulposus Explant
نویسندگان
چکیده
منابع مشابه
The Regenerative Potential of Notochordal Cells in a Nucleus Pulposus Explant
STUDY DESIGN In vitro disk explant culture. OBJECTIVE Notochordal cells (NCs) have been shown to upregulate matrix production by nucleus pulposus (NP) cells in coculture. To examine the translation of these in vitro results to a nativelike setting, the regenerative potential of NCs injected into NP tissue was assessed in this study. METHODS NP explants were cultured after injection with NCs...
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Many of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro with or without chondrogenic reagents for differen...
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During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for these effects, we characterized NC-derived extracellular vesicles (EVs) and determined their effect on CLCs. EVs were purified from porcine NC-conditioned medium (NCCM) through size exc...
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pulposus phenotype in human MSCs +Purmessur, D; Godburn K; Abbott, RD: Schek RM; Iatridis JC +University of Vermont, Burlington VT Senior author [email protected] INTRODUCTION Mesenchymal stem cells (MSCs) are an attractive cell source for IVD repair, yet the means of differentiating these cells to a nucleus pulposus (NP) phenotype is not clear and an area of high priority. Notochordal cel...
متن کاملChordoma‐derived cell line U‐CH1‐N recapitulates the biological properties of notochordal nucleus pulposus cells
Intervertebral disc degeneration proceeds with age and is one of the major causes of lumbar pain and degenerative lumbar spine diseases. However, studies in the field of intervertebral disc biology have been hampered by the lack of reliable cell lines that can be used for in vitro assays. In this study, we show that a chordoma-derived cell line U-CH1-N cells highly express the nucleus pulposus ...
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ژورنال
عنوان ژورنال: Global Spine Journal
سال: 2017
ISSN: 2192-5682,2192-5690
DOI: 10.1055/s-0036-1583174